Novel Human C3 Derivatives for Therapeutic Complement Depletion - Complement depletion as a novel therapeutic approach, with applications in treating diseases with complement pathology

Case ID: 00985

Description

The complement system is part of the immune system and is important in both innate and adaptive immunity. However, the complement system can also be a pathogenetic factor in many diseases including, rheumatoid arthritis, reperfusion injury, myasthenia gravis, and more. Complement has also been shown to be important for the development of antibodies to biological therapeutics such as human Factor VIII in hemophilia A.

 

The development of agents to interfere with or modulate the complement cascade has been an area of intense interest for the past two decades. Furthermore, the FDA recently issued a guidance for the adoption of a risk-based approach to evaluating and mitigating immunogenicity associated with therapeutic proteins.

 

A novel therapeutic approach has been applied to complement pathogenesis: complement depletion. The approach came out of years of research with cobra venom factor (CVF), a C3 analog known to be able to deplete complement. Utilizing knowledge of the structure and function of CVF and the C3 component of complement, the researchers created derivatives of human C3 (which they called humanized CVF or hCVF) which display CVF-like activity in depleting complement without displaying toxicity.

 

These hCVF C3 derivatives were tested for therapeutic complement depletion in multiple preclinical models of diseases with complement pathology, including reperfusion injury, age-related macular degeneration (AMD), paroxysmal nocturnal hemoglobinuria (PNH), and immunogenicity to Factor VIII in hemophilia A and consistently showed therapeutic efficacy. Chinese hamster ovary (CHO) cell lines have been developed for gram quantity production of the hCVF C3 derivatives. 

 

Applications

 

Treatment of chronic diseases with complement involvement

  • Rheumatoid Arthritis
  • Age-related Macular Degeneration
  • Myasthenia Gravis
  • Paroxysmal Nocturnal Hemoglobinuria
  • Atypical Hemolytic-Uremic Syndrome

 

Acute treatments with complement involvement

  • Reperfusion injury
  • Ventilator-induced lung Injury
  • Angioplasty
  • mAb therapy of lymphoma

 

Immunogenicity from biologic therapeutics

  • Hemophilia A
  • Other blood clotting conditions associated with unwanted complement activation

 

Advantages

 

These C3 derivatives induce complement depletion without toxicity, even after intra-arterial injection into the pulmonary artery of primates. No immunogenicity, to date, has been observed. As such, these derivatives would be excellent candidates for clinical applications requiring short-term complement depletion, even on a repeated basis.

 

Further Details

 

Vogel C. W. et al., Mol. Immunol. 2014 Oct; 61(2):191-203.

 

Patents

 

Granted US Patent (8,632,780) and Japan 6488376. Other applications pending.

Patent Information:
Inventors
Carl-Wilhelm Vogel
Paul Finnegan
Julie Rayes
Sebastien Lacroix-Desmazes

For information, contact:
Ken Takeuchi
Technology Licensing Associate
University of Hawaii
(808) 956-9749
kktakeuc@hawaii.edu
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Healthcare
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